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γ -secreatase inhibitor dapt  (Millipore)
90
Millipore γ -secreatase inhibitor dapt
Expression of dnMAML in MCF-7 cells. (A) Western blot of cell lysate from MCF7-GFP and MCF7-dnMAML cells. The blot was probed with anti-GFP, stripped, and reprobed with antitubulin. (B) Comparison of HES1 levels between DMSO- and <t>DAPT-treated</t> MCF-7 as well as comparison between MCF7-GFP and MCF7-dnMAML cells, as quantified with RT-qPCR. (C) Relative levels of HEY family members and HES1 quantified with RT-qPCR in MCF-7 cells after 48 h of coculture. The expression levels were normalized to MCF-7 cells cocultured with SVEC-MIY cells (without Jagged-1 overexpression). Error bars in (B and C) are standard errors of the mean. (D) Percentage of GFP + cells traced over a period of 30 days using flow cytometry. Results from two independent cell transductions are shown.
γ Secreatase Inhibitor Dapt, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B3+-secretase+inhibitor+dapt/%CE%B3++secreatase+inhibitor+dapt/pmc03544441-56-13-17
Average 90 stars, based on 1 article reviews
γ -secreatase inhibitor dapt - by Bioz Stars, 2026-10
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Expression of dnMAML in MCF-7 cells. (A) Western blot of cell lysate from MCF7-GFP and MCF7-dnMAML cells. The blot was probed with anti-GFP, stripped, and reprobed with antitubulin. (B) Comparison of HES1 levels between DMSO- and DAPT-treated MCF-7 as well as comparison between MCF7-GFP and MCF7-dnMAML cells, as quantified with RT-qPCR. (C) Relative levels of HEY family members and HES1 quantified with RT-qPCR in MCF-7 cells after 48 h of coculture. The expression levels were normalized to MCF-7 cells cocultured with SVEC-MIY cells (without Jagged-1 overexpression). Error bars in (B and C) are standard errors of the mean. (D) Percentage of GFP + cells traced over a period of 30 days using flow cytometry. Results from two independent cell transductions are shown.

Journal: Cancer Medicine

Article Title: Heterogeneity of breast cancer stem cells as evidenced with Notch-dependent and Notch-independent populations

doi: 10.1002/cam4.18

Figure Lengend Snippet: Expression of dnMAML in MCF-7 cells. (A) Western blot of cell lysate from MCF7-GFP and MCF7-dnMAML cells. The blot was probed with anti-GFP, stripped, and reprobed with antitubulin. (B) Comparison of HES1 levels between DMSO- and DAPT-treated MCF-7 as well as comparison between MCF7-GFP and MCF7-dnMAML cells, as quantified with RT-qPCR. (C) Relative levels of HEY family members and HES1 quantified with RT-qPCR in MCF-7 cells after 48 h of coculture. The expression levels were normalized to MCF-7 cells cocultured with SVEC-MIY cells (without Jagged-1 overexpression). Error bars in (B and C) are standard errors of the mean. (D) Percentage of GFP + cells traced over a period of 30 days using flow cytometry. Results from two independent cell transductions are shown.

Article Snippet: Induction of Notch targets in the coculture experiment was compared between treatment with γ -secreatase inhibitor DAPT (Calbiochem, San Diego, CA) and carrier control DMSO (Sigma-Aldrich, St. Louis, MO).

Techniques: Expressing, Western Blot, Comparison, Quantitative RT-PCR, Over Expression, Flow Cytometry